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n terminal mcherry fusion vector  (TaKaRa)


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    TaKaRa n terminal mcherry fusion vector
    N Terminal Mcherry Fusion Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 3393 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/n+terminal+mcherry+fusion+vector/pmCherry-N1+Vector/pm33368703-77-24-30
    Average 96 stars, based on 3393 article reviews
    n terminal mcherry fusion vector - by Bioz Stars, 2026-09
    96/100 stars

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    Plasmid Preparation:

    Article Title: Kif3a deletion prevents primary cilia assembly on oligodendrocyte progenitor cells, reduces oligodendrogenesis and impairs fine motor function
    Article Snippet: Protein expression was assessed using rabbit anti‐Kif3a (1:2000, Abcam ab11259) and mouse anti‐β‐actin (1:15000, Sigma A2228) detected with anti‐rabbit HRP (1:5000, Dako P0448) and anti‐mouse HRP (15,000, Dako P0447), respectively. .. The pEGFP‐Arl13b plasmid was generously donated by Judith Paridaen and Weiland Huttner (Max Planck Institute, Dresden, Germany; (Paridaen, Wilsch‐Brauninger, & Huttner, ) and the N‐terminal mCherry fusion vector purchased from Clontech (632523; Mountain View, CA). .. For transformations, DSHΔ chemically competent Escherichia coli were incubated with 500 ng of plasmid DNA in ice‐cold KCM (10 mM KCl, 30 mM CaCl 2 , 50 mM MgCl 2 ) at 42°C for 60 s, before being rapidly transferred to ice for a further 5 min. 1 ml of antibiotic‐free luria broth was added, and after 45 min at 37°C, the bacteria were transferred to agar plates containing 100 μg/ml kanamycin (Thermo Fisher) and incubated at 37°C overnight.

    Article Title: Kif3a deletion prevents primary cilia assembly on oligodendrocyte progenitor cells, reduces oligodendrogenesis and impairs fine motor function.
    Article Snippet: Protein expression was assessed using rabbit anti-Kif3a (1:2000, Abcam ab11259) and mouse anti-β-actin (1:15000, Sigma A2228) detected with anti-rabbit HRP (1:5000, Dako P0448) and anti-mouse HRP (15,000, Dako P0447), respectively. .. The pEGFP-Arl13b plasmid was generously donated by Judith Paridaen and Weiland Huttner (Max Planck Institute, Dresden, Germany; (Paridaen, Wilsch-Brauninger, & Huttner, 2013) and the N-terminal mCherry fusion vector purchased from Clontech (632523; Mountain View, CA). .. For transformations, DSHΔ chemically competent Escherichia coli were incubated with 500 ng of plasmid DNA in icecold KCM (10 mM KCl, 30 mM CaCl2, 50 mM MgCl2) at 42 C for 60 s, before being rapidly transferred to ice for a further 5 min. 1 ml of antibiotic-free luria broth was added, and after 45 min at 37 C, the bacteria were transferred to agar plates containing 100 μg/ml kanamycin (Thermo Fisher) and incubated at 37 C overnight.



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